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| Registros recuperados: 32 | |
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| Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del. |
| Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308 |
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| Desvignes, T.; Fostier, A.; Fauvel, Christian; Bobe, J.. |
| The Nme gene family, also known as Nm23 or NDPK, is a very ancient gene family that can be found in all kingdoms of life. In the late eighties, a gene of the Nme family, NME1, was identified as the first metastatic suppressor gene, resulting in a major interest for this family. Due to the complexity of the family, the need for a unified and evolutionary-supported gene nomenclature was recently stressed by the scientific community. Based on a complete evolutionary history study of the gene family in metazoans and vertebrates, a unified nomenclature was recently proposed and accepted by gene nomenclature consortia. In addition to its well-documented role in tumor metastasis, members of the Nme family are also involved in a wide variety of cellular and... |
| Tipo: Text |
Palavras-chave: Nm23; NDPK; RP2; Teleost; Oocyte; Ovary. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00133/24466/22504.pdf |
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| Desvignes, Thomas; Nguyen, Thaovi; Chesnel, Franck; Bouleau, Aurelien; Fauvel, Christian; Bobe, Julien. |
| Retinitis pigmentosa 2 (RP2) gene is responsible for up to 20% of X-linked retinitis pigmentosa, a severe heterogeneous genetic disorder resulting in progressive retinal degeneration in humans. In vertebrates, several bodies of evidence have clearly established the role of Rp2 protein in cilia genesis and/or function. Unexpectedly, some observations in zebrafish have suggested the oocyte-predominant expression of the rp2 gene, a typical feature of maternal-effect genes. In the present study, we investigate the maternal inheritance of rp2 gene products in zebrafish eggs in order to address whether rp2 could be a novel maternal-effect gene required for normal development. Although both rp2 mRNA and corresponding protein are expressed during oogenesis, rp2... |
| Tipo: Text |
Palavras-chave: Developmental biology; Egg developmental competence; Egg quality; Fish; Fish reproduction; Maternal-effect gene; Ndpk; Nme10; Oocyte; Oocyte-specific; Ovum; Teleost; Zygote. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00280/39099/37660.pdf |
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| De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud. |
| A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment... |
| Tipo: Text |
Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00260/37147/35676.pdf |
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| Comtet, T; Garcia, Celine; Le Coguic, Yvette; Joly, Jean-pierre. |
| We report the occurrence of the microsporidian parasite Steinhausia sp. in the oocytes of the common cockle Cerastoderma edule in a natural population in France, where high mortalities occurred. Steinhausia sp. appeared primarily as sporocysts containing many small spores, and putative earlier developmental stages were also observed. Both its prevalence and infection intensity were low, and no host defence reaction was recognized, suggesting that Steinhausia sp. had no detrimental effect on C. edule. Its prevalence was higher in cockles lying on the sediment surface, but the significance of this observation could not be explained given the poor knowledge of the Steinhausia life cycle. The present data did not allow specific identification of the parasite,... |
| Tipo: Text |
Palavras-chave: Cerastoderma edule; Steinhausia sp.; Microsporida; Oocyte; Baie des veys. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00000/10762/7586.pdf |
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| Desvignes, T.; Fauvel, Christian; Bobe, J.. |
| After the recent report of the expression of several nme genes in the zebrafish gonads, the present study aimed at further analyzing the expression of nme genes in the ovary with special attention for the nme transcripts that are maternally inherited and could thus participate in the determination of oocyte developmental competence. The expression levels of all groups I and II nme genes were characterized by QPCR in a panel of zebrafish tissues. The nme genes exhibiting an ovarian expression were subsequently monitored throughout oogenesis and early development, and their expression sites characterized using in situ hybridization. Here, we show that nme2b1, nme3, nme4, and nme6 are highly expressed in the ovary and present in the zebrafish oocyte... |
| Tipo: Text |
Palavras-chave: NDPK; Nm23; Oocyte; Maternal; Teleost; Vertebrate. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16226/13778.pdf |
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| Vignier, J.; Volety, A. K.; Rolton, A.; Le Goic, N.; Chu, F. -l. E.; Robert, Rene; Soudant, P.. |
| The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF. Following a 30-min exposure, the number of spermatozoa and their viability were... |
| Tipo: Text |
Palavras-chave: Deepwater Horizon oil spill; Oyster; Spermatozoa; Oocyte; Flow-cytometry. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00377/48851/51168.pdf |
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| Ferreira,M. A. P.; Paixão,L. F.; Alcântara-Neto,C. P.; Santos,S. S. D.; Rocha,R. M.. |
| Crassostrea rhizophorae (Guilding, 1828) is one of the tropical species of oysters cultivated on the Brazilian shore. Despite its high commercial value, there is no information on the gonadal characteristics during the reproductive stages of this species. The objective of this work was to analyze the morphology and morphometry of Crassostrea rhizophorae oocytes and follicles in three stages of the gonadal cycle. Were analized histological sections of gonads considering that the oocytes presented visible nuclei and nucleoli, it was observed three gonadal cycle stages: early gametogenesis, growth and maturation. In the early gametogenesis stage, follicles and oocytes presented diameters of 180.29 (± 41.91) and 18.66 (± 6.85) µm, respectively, and presence of... |
| Tipo: Journal article |
Palavras-chave: Crassostrea; Oocyte; Gonad; Morphology. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400023 |
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| Esponda,Pedro. |
| The production of transgenic animals (TA) using transfected spermatozoa or eggs is commented. Different methods have been employed to introduce transgenes into the gametes of several vertebrates and invertebrates. Methods for the transfection of gametes have employed naked DNA, viral vectors, DNA/Liposome complexes, electroporation or high velocity microprojectiles. Spermatozoa and oocytes or eggs have showed a good transfection efficience (80% in some cases), and microscopical observations demonstrated that the transgenes appeared localized in the nucleus. Gametes have shown to be naturally protected against the entrance of foreign genes because some semino plasma or plasma membrane proteins block the entrance of foreign genes in spermatozoa. In the... |
| Tipo: Journal article |
Palavras-chave: Eggs; Oocyte; Spermatozoa; Transfection; Transgenic animals. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300017 |
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| Haque,Ziaul; Haque,Azimul; Quasem,Md. Abul. |
| Ovary plays the vital role in the reproductive biology and biotechnology of female animals. With the aim to study the ovarian morphometry of Black Bengal goat, both right and left ovaries were collected from the slaughter houses of different Thanas under Mymensingh district. For each of the specimens, gross parameters such as weight, length and width were recorded. Then they were processed and stained with H & E for histomorphometry. Our study revealed that the right ovary (0.53±0.02 g) was heavier than the left (0.52±0.02 g). The length of the right ovary (1.26±0.04 cm) was lower than the left (1.28±0.02 cm) but the width of the right (0.94±0.02 cm) was greater than the left (0.90±0.03 cm). The diameter of ovarian follicles in the cortex was... |
| Tipo: Journal article |
Palavras-chave: Morphometry; Ovarian follicles; Cortex; Medulla; Oocyte. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100002 |
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| Fortes,P; Salvador,G; Cônsoli,FL. |
| Ovary development and maturation of Nezara viridula (L.) were evaluated by examining ovariole morphology and the alterations in the biochemical (protein synthesis related to reproduction) composition of the hemolymph. Quantitative and qualitative protein analyses were performed and ovary structural alterations for the pre-reproductive and reproductive stages were recorded. Total concentration of proteins in female hemolymph gradually increased until the end of the pre-mating stage, remaining unaltered thereafter. Proteins linked to reproduction (vitellogenins) appeared in the hemolymph 10 days after adult emergence and indicated the end of the pre-mating stage. After mating, total protein concentration in the hemolymph was lower compared to virgin females;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitellogenesis; Reproduction; Oocyte. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000100013 |
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| Araújo,Francisco G.; Nascimento,Aparecida A.; Gomes,Iracema D.; Sales,Armando; Oliveira,Beatriz A. Chagas de. |
| ABSTRACT The gonadal development of the characin Astyanax aff. bimaculatus from Funil Reservoir, an impoundment in the middle reaches of the Paraiba do Sul River in southeastern Brazil was analysed through histological, histochemical and histometric techniques, and the spawning season was determined. Unbalanced sex ratio was found, with females outnumbering and reaching larger sizes than males. The following stages of oogenic cell development were identified for females: primary growth, pre-vitellogenic (cortical alveolar) and vitellogenic. In males, the following stages were determined: spermatogonia primary and secondary, spermatocyte primary and secondary, spermatid and spermatozoa. The spawning occurs most of the year, peaking in January-February, as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fish; Histology; Oocyte; Spawning type; Spermatocyte. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702019000100326 |
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| Bombardelli,Robie Allan; Sanches,Eduardo Antônio; Baggio,Diego Mendes; Sykora,Renata Marino; Souza,Bruno Estevão de; Tessaro,Lucélia; Piana,Pitágoras Augusto. |
| The objective of this study was to evaluate the effects of water volume and water temperature on the sperm motility duration and the number of spermatozoa, and the water volume on the fertilization rates of oocytes of Rhinelepis aspera. Experiments were carried out to evaluate the effect of semen dilutions (1.74×10-5, 1.74×10-4, 1.74×10-3, 1.74×10-2, 1.74×10-1 and 1.00 mL of sperm.mL-1 of water) and water temperature (5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 ºC) on spermatozoa motility duration. In addition, the effects of insemination dose (7×10³, 7×10(4), 7×10(5), 7×10(6) and 7×10(7) spermatozoa.oocyte-1) and water volume (1.0, 30.0, 60.0, 90.0 and 120.0 mL water.2.0 mL-1 oocytes) on the artificial fertilization rates of oocytes were evaluated. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Loricariidae; Oocyte; Reproduction; Semen. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000100001 |
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| Britt,Jack H.. |
| Oocytes in cattle are formed during embryogenesis and develop within individual follicles in the cortex of the ovary. Dormant primordial follicles become active and undergo progressive development at regular intervals commencing during the late fetal stage and continuing throughout adulthood. Once activated, follicles and oocytes in a cohort either grow to maturation and ovulation or undergo atresia, ultimately depleting the ovaries of germ cells. It takes an estimated 100 days for a follicle and its oocyte to reach the mature ovulatory stage. Within an individual, number of follicles in one ovary is similar to number in the other ovary; however, there are large differences among individuals in total number of follicles present in both ovaries. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Follicle; Ovary; Oocyte; Embryo; Fecundity. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300013 |
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| Sá,M.A.F.; Paiva,S.O.; Dutra,G.A.; Barbosa,C.G.; Mello,M.R.B.; Jacob,J.C.F.. |
| ABSTRACT The success of transvaginal follicular aspiration in mares can be influenced by several factors, such as vacuum pump pressure levels. The present study aimed to investigate the effect of different negative pressures (150, 280 and 400mmHg) of the vacuum pump on the oocyte recovery in the mares. The mares (n=10) were undergoing follicular aspiration using three different negative pressures for three consecutive estrous cycles as follows: G150 = 150mmHg (n = 10); G280 = 280mmHg (n = 10); G400 = 400mmHg (n = 10). Every estrous cycle, the group that the mare would participate was drawn, and each animal participated once in each group. Only preovulatory follicle was used, about 30 to 36 hours after application of hCG. To compare the results, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oocyte; Subfertility; Preovulatory follicle; HCG. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300529 |
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| Registros recuperados: 32 | |
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